000			04055nab a22003737a 4500
003			EC-PaCDF
005			20171005141651.0
008			160817t20162016xxu|||||||||||||||||eng||
040			_aEC-PaCDF
041			_aeng
082	0	4	_a598.857 _221
092	0		_a598.857 _bLAH _221
100	1		_aLahuatte, Paola F.
245	1	0	_aRearing larvae of the avian nest parasite, Philornis downsi (Diptera: Muscidae), on chicken blood-based diets.
260			_c2016
520	0		_aCaptive rearing of insect pests is necessary to understand their biology and to develop control methods. The avian nest fly, Philornis downsi Dodge and Aitken, is a blood-sucking parasite during its larval stage and a serious threat to endemic birds in the Galapagos Islands where it is considered invasive. In order to procure large numbers of flies for biological studies, rearing media and diets were trialed for rearing the larval stage of P. downsi under controlled conditions in the absence of its avian host. P. downsi eggs were obtained from fieldcaught female flies, and once eggs hatched they were reared on chicken blood for the first 3 d. Following this, three diets were tested on second- and third-instar larvae: 1) chicken blood only; 2) chicken blood, hydrolyzed protein and dried milk powder; and 3) chicken blood, hydrolyzed protein and brewer’s yeast. Out of 385 P. downsi larvae tested, we were able to rear 50 larvae to the adult stage. The highest level of mortality was found in the first-instar larvae. Survivorship of second- and third-instar larvae was similar irrespective of diet and diet did not significantly influence larval or pupal development times; though larvae fed the diet with brewer’s yeast developed marginally faster. Pupal weights were similar to those of larvae that had developed on bird hosts in the field. To our knowledge, this is the first effective protocol for rearing a hematophagous parasitic avian fly from egg to adult in the absence of a living host. Captive rearing of insect pests is necessary to understand their biology and to develop control methods. The avian nest fly, Philornis downsi Dodge and Aitken, is a blood-sucking parasite during its larval stage and a serious threat to endemic birds in the Galapagos Islands where it is considered invasive. In order to procure large numbers of flies for biological studies, rearing media and diets were trialed for rearing the larval stage of P. downsi under controlled conditions in the absence of its avian host. P. downsi eggs were obtained from fieldcaught female flies, and once eggs hatched they were reared on chicken blood for the first 3 d. Following this, three diets were tested on second- and third-instar larvae: 1) chicken blood only; 2) chicken blood, hydrolyzed protein and dried milk powder; and 3) chicken blood, hydrolyzed protein and brewer’s yeast. Out of 385 P. downsi larvae tested, we were able to rear 50 larvae to the adult stage. The highest level of mortality was found in the first-instar larvae. Survivorship of second- and third-instar larvae was similar irrespective of diet and diet did not significantly influence larval or pupal development times; though larvae fed the diet with brewer’s yeast developed marginally faster. Pupal weights were similar to those of larvae that had developed on bird hosts in the field. To our knowledge, this is the first effective protocol for rearing a hematophagous parasitic avian fly from egg to adult in the absence of a living host.
546			_aEnglish
653			_aRearing.
653			_aCría.
653			_aLarvae.
653			_aLarvas.
653			_aParasite.
653			_aParásito.
653			_aNest.
653			_aNido.
653			_aPhilornis downsi.
653			_aDiptera Muscidae
700	1		_aLincango, M.
700	1		_aHeimpel, G.
700	1		_aCauston, C. _91525
773	0		_tJournal of Insect Science _gVol.16, no, 1 (Jun 2016), p. 1-7.
856	4	0	_uhttp://dx.doi.org/10.1093/jisesa/iew064
942			_2ddc _cARTICLE
999			_c12747 _d12747